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Five additional groups were vaccinated three times at three-week intervals with the following formulations: AddaS03™ adjuvant at a 1:1 volume ratio in a total of 100 µL PBS/mouse, AS01 adjuvant (5 µg MPL/5 µg QS-21 in a total of 100 µL PBS/mouse), metE-DNA (100 µg/100 µL PBS/mouse), 1 µg metE protein combined with AddaS03™ adjuvant at a 1:1 volume ratio in a total of 100 µL PBS/mouse, and AS01 adjuvant (5 µg MPL/5 µg QS-21 in a total of 100 µL PBS/mouse)

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Arginine is involved in the production of nitric oxide (NO), a molecule that plays a key role in immune signaling and pathogen defense

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