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Minimal differences were observed in the nucleotide metabolism and the TCA cycle between aged Mda5 -/- and WT HSCs (Supplementary Fig

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1X SSC = 150 mM NaCl and 15 mM sodium citrate), and hybridized in riboprobe solution (riboprobe diluted in Hyb solution: 50% deionized formamide, 0.5X SSC, 1% Tween-20 in Diethyl Pyrocarbonate (DEPC)-treated water) with riboprobes diluted to between 1:800 and 1:4000 depending on the probe strength