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2.2.1 Total phenols, flavonoids, and antioxidant profiling Total phenol content was estimated via the spectrophotometric method using Folin Ciocalteu’s Reagent (FCR) ( 3 and NaNO 2 at alkaline pH ( 2.2.2 Amino acid profiling For lysine estimation, 100 mg of finely ground sample was taken, to which 5 ml of papain was added and incubated overnight at 65°CC
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