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Contrary to alternative method based on a ‘competitive’ hypothesis, GSVA is based on ‘self-contained null hypothesis’ which analyzes each gene set in isolation, assessing differential expression of the gene set without comparing to a background [62, 63]
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In addition, it was found that orally administered luminal antigens were taken up by the Cld4 + EECs, raising the possibility that EECs may also play a role in initiation of mucosal immunity 162
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