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Materials and methods Cell culture and compound treatment HepG2 cells (ATCC, catalog: HB-8065) were cultured with 90% Eagle’s Minimum Essential Medium (EMEM, ATCC, catalog: 30-2003) and supplemented with 10% fetal bovine serum (FBS, Cytiva, catalog: SH30070.03) and 100 U/mL penicillin-100 µg/mL streptomycin (Thermo Fisher Scientific, catalog: 15140122)

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In future trials it will be important to keep track of comorbidities for clinical trial design, either for the purpose of post hoc subgroup analysis or a priori subgroup recruitment, since the neuroathophysiological mechanisms in IC/ BPS patients with different co-morbidities are likely to be different

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