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The tripeptide architecture, the gamma-bond, the thiol working surface, the cycling enzymes — the full chemical package, in some lineages, has remained essentially unchanged for what the field describes as a substantial fraction of the entire evolutionary history of cellular life
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coli BL21 (DE3) (Sigma-Aldritch) at ≥ 110-fold coverage of the library size, and following 1-hr recovery from transformation, cells were immediately diluted into LB media (Gibco) containing kananycin (selecting for presence of the library) and 10 uM IPTG (inducer for library expression), beginning the massively parallel inhibition measurements
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