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Total RNA was isolated from SH-SY5Y cells treated overnight (20 h) with morphine (100 nM), D-CYSee (100 μM, betaine (300 μM), morphine (100 nM) + D-CYSee (100 μM) or morphine (100 nM) + betaine (300 μM) using the TRIzol reagent ( Thermo Fischer Scientific , Waltham, MA)
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