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Flow cytometry and cell sorting For analysis of spinal cord cell populations, stainings were performed in PBS using fixable viability dye (Invitrogen, 65-0865-14, 1:2,000, 10 min at room temperature) as well as the following antibodies: CD45.2-BV650 (clone 104, Biolegend, 109836, 1:100), Ly6G-BV605 (clone 1A8, Biolegend, 127639, 1:100), Ly6G-AF700 (clone 1A8, Biolegend, 127622, 1:100), CD3-BV605 (clone 145-2C11, Biolegend, 100351, 1:100), Ly6C-BV605 (clone HK14, Biolegend, 128035, 1:100), Ly6C-BV510 (clone HK14, Biolegend, 128033, 1:100), CD11b-BV510 (clone M1/70, Biolegend, 101245, 1:100), CD11b-PB (clone M1/70.15, Invitrogen, RM2828, 1:100), CD11b-BV605 (clone M1/70, Biolegend, 101237, 1:100), CX3CR1-PeCy7 (clone SA011F11, Biolegend, 149015, 1:100), CCR2-BV421 (clone SA203G11, Biolegend, 150605, 1:100), CCR2-FITC (clone SA203G11, Biolegend, 150607, 1:100), F4/80-BV510 (clone BM8, Biolegend, 123135, 1:100), F4/80-BV605 (clone BM8, Biolegend, 123133, 1:100), F4/80-FITC (clone BM8, Biolegend, 123108, 1:100), CD11c-PE-Cy5.5 (clone N418, Invitrogen, 35-0114-82, 1:100), P2RY12-APC (clone S16007D, Biolegend, 848006, 1:100), P2RY12-AF488 (clone S16007D, Biolegend, 848016, 1:100), MerTK-APC (clone 2B10C42, Biolegend, 151507, 1:100), CD44-FITC (clone IM7, Invitrogen, 11-0441-82, 1:100), CD44-eF506 (clone IM7, Invitrogen, 69-0441-82, 1:100), CD64-BV605 (clone X54-5/7.1, Biolegend, 139323, 1:100), CD3-AF700 (clone 17A2, Biolegend, 100216, 1:100), CD3-rF710 (clone 17A2, Tonbo, 80-0032-U100, 1:80), CD8-BV510 (clone 53-6.7, Biolegend, 100751, 1:80), CD4-APC (clone REA604, Miltenyi Biotec, 130-116-487, 1:80), CD4-PE (clone REA604, Miltenyi Biotec, 130-116-509, 1:80) and CD25-BV421 (clone PC61, Biolegend, 102033, 1:80)

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In conclusion, systemic application of bpc 157 does not alter blood circulation in healthy gingiva