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The sample supernatant was detected with reaction buffer (50 mM Tris‒HCl, pH 8.0, 150 mM NaCl, 1 mM CaCl, 0.1 mg/mL bovine serum albumin [BSA]) containing Boc‐Gln‐Ala‐Arg‐MCA (Peptide, 3135‐v) at excitation wavelength of 380 nm and emission wavelength of 440 nm using a plate reader (CLARIOstar, BMG Labtech) for 10 min

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Size:10 Tablets

The earliest studies of MOR phosphorylation established that the receptor is phosphorylated more efficiently following activation by high-efficacy agonists, such as DAMGO or etorphine, than by low-efficacy agonists such as morphine in cell lines (Yu et al., 1997