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Briefly, cells were lysed with 1X lysis buffer (Cell Signalling Technology, #: 9803S), and lysates were centrifuged at 17,000 g at 4 °C for 10 min

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Meanwhile, 25-OHC markedly upregulated cytochrome b5 reductase 1 (CYB5R1) and POR, suppressed GSH expression, and promoted lipid peroxidation, thereby enhancing the sensitivity of IMS32 cells to ferroptosis and aggravating neuroglial injury

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