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Therefore, we utilized MEFs with endogenous, homozygous knock-in of the elF2α -S5IA mutation ( eIF2α A/A ), which fail to induce ATF4 downstream of cellular stress (Figure supplement 2B) (Scheuner et al., 2001)
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Animal studies using IT infused or inhaled H confirmed the protective value of NAC administered immediately after or 30 min after H exposure
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