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ACTL6A impacts GSH de novo synthesis mainly by upregulating γ-glutamyl-cysteine synthesis To further assess the mechanism by which ACTL6A regulates GSH metabolism, we investigated the metabolic fate of U- 13 C glucose by liquid chromatography with mass spectrometry (LC–MS), which produces γ-GC containing two 13 C atoms via GSH de novo synthesis pathway, GSH containing two 13 C atoms via GSH de novo synthesis or the SGOC pathway, or four 13 C atoms via both pathways (Fig

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Clinical and experimental evidence also suggests potential hepatic and renal complications: rodent studies have demonstrated inflammatory and vascular changes in the liver after prolonged KD exposure [95], while in humans, elevated nitrogen excretion and hyperfiltration have been linked to an increased risk of nephrolithiasis [96]