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For qPCR, the IQ5 real-time PCR detection system (Bio-Rad) was performed using Absolute Blue qPCR Mastermix with SYBR Green (Thermo Fisher Scientific) and the following cycle parameters: Cycle 1 (95 °C for 15 min), Cycle 2 (95 °C for 15 s), Cycle 3 (58 °C for 30 s), Cycle 4 (72 °C for 30 s), repeat Cycle 2 to 4 for 39 times, Cycle 5 (95 °C for 30 s), Cycle 6 (Melt Curve 60 °C to 100 °C), Cycle 7 (4 °C forever)

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(2017) showed that GW3965 (a LXR agonist) treatment significantly increased ABCA1 level and increased neurite/axonal outgrowth in vitro , which helps to verify the conclusion above